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Journal: iScience
Article Title: Crosstalk between circulating DPP3 and immune cells in the context of cardio-systemic stress
doi: 10.1016/j.isci.2026.115114
Figure Lengend Snippet: A fraction of DPP3 is transported via EVs and upregulated during septic shock in humans (A) Western blot qualitative analysis of cluster of differentiation 63 (CD63) at 26 KD (bottom), heat shock protein 70 (HSC70) at 73 KD (middle), and DPP3 at 83 KD (above), in protein extracts of EVs derived from bone marrow supernatant (BM SN) from Ctrl mice (left) and ISO mice (right). DPP3 activity (U/L) in large (lEVs) and small (sEVs) EVs derived from (B) plasma of control mice (Ctrl) ( n = 6) and isoproterenol-treated mice (ISO) ( n = 5), or from (C) BM SN of Ctrl ( n = 6) and ISO mice ( n = 6). (D) DPP3 activity in lEVs and sEVs derived from BM SN of WT mice injected with bone marrow cells from Dpp3-KO (WT BM KO ) and Dpp3-KO mice injected with bone marrow cells from WT mice (KO BM WT ) ( n = 5) for each. (E) Quantification of Nano track analysis (NTA) results of plasma derived-lEVs and sEVs concentration (vesicle/ml) in healthy individuals ( n = 7), septic shock (SS) ( n = 6) and cardiogenic shock (CS) patients ( n = 5). (F) DPP3 activity (U/L) in lEVs and sEVs derived from plasma of healthy individuals ( n = 8) and septic shock (SS) patients ( n = 6). (G) DPP3 activity (U/L) in lEVs and sEVs derived from plasma of healthy individuals ( n = 8) and cardiogenic shock (CS) patients ( n = 11). DPP3 activity (U/L) in lEVs and sEVs derived from cell culture supernatant of (H) human cardiomyocytes AC16 ( n = 6) and (I) human monocytes THP-1 ( n = 2). In all bars, data are presented as mean ± standard error of the mean (SEM). Comparisons were made by Multiple Mann-Whitney tests in B, C, F, and G, and by two-way ANOVA in D and E. Significance was presented as follows (∗ p < 0.05, ∗∗ p < 0.01, and ns for non-significant differences).
Article Snippet:
Techniques: Western Blot, Derivative Assay, Activity Assay, Clinical Proteomics, Control, Injection, Concentration Assay, Cell Culture, MANN-WHITNEY